Hi All, I'm trying to use genion to insert 7 Na counter ions into a protein simulation. I use the following command line. genion -s md.tpr -np 7 -pq 1 -o withNa.gro This works as it should and replaces 7 water molecules with Na. I then fix the topology file by subtracting 7 from the number of solvent molecules and add 7 Na molecules. I also add ions.itp. The problem occurs when I try to run these new .gro and .top files through grompp. I get the error: Fatal error: No such moleculetype Na This error seems a little strange since the molecule type Na is define in ions.itp. Let me know if you can tell what I'm doing wrong. Thanks Alot. Erik Storm