<HTML><BODY style="word-wrap: break-word; -khtml-nbsp-mode: space; -khtml-line-break: after-white-space; "><P class="MsoNormal" style="mso-pagination:none;tab-stops:28.0pt 56.0pt 84.0pt 112.0pt 140.0pt 168.0pt 196.0pt 224.0pt 3.5in 280.0pt 308.0pt 336.0pt; mso-layout-grid-align:none;text-autospace:none"><SPAN style=""><SPAN class="Apple-style-span" style="font-size: 24px;"><FONT class="Apple-style-span" face="Chalkboard"><FONT class="Apple-style-span" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">Hi all, </SPAN></FONT></FONT></SPAN></SPAN><SPAN style=""><FONT class="Apple-style-span" size="6"><SPAN class="Apple-style-span" style="font-size: 24px;"><FONT class="Apple-style-span" face="Century"><FONT class="Apple-style-span" face="Calisto MT"><FONT class="Apple-style-span" face="Century Gothic"><FONT class="Apple-style-span" face="Century Schoolbook"><FONT class="Apple-style-span" face="Chalkboard"><FONT class="Apple-style-span" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"><O:P></O:P></SPAN></FONT></FONT></FONT></FONT></FONT></FONT></SPAN></FONT></SPAN></P><DIV class="MsoNormal"><SPAN style=""><SPAN class="Apple-style-span" style="font-size: 24px;"><FONT class="Apple-style-span" face="Chalkboard"><FONT class="Apple-style-span" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"> I wonder if someone used the new GROMOS force field (53a) to simulate lipids? I wish to know your opinion regarding using it with DMPC/PEPTIDE system, also which one 5 or 6 is better to membrane systems.</SPAN></FONT></FONT></SPAN><FONT class="Apple-style-span" size="6"><SPAN class="Apple-style-span" style="font-size: 24px;"><FONT class="Apple-style-span" face="Century"><FONT class="Apple-style-span" face="Calisto MT"><FONT class="Apple-style-span" face="Century Gothic"><FONT class="Apple-style-span" face="Century Schoolbook"><FONT class="Apple-style-span" face="Chalkboard"><FONT class="Apple-style-span" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"><O:P></O:P></SPAN></FONT></FONT></FONT></FONT></FONT></FONT></SPAN></FONT></SPAN></DIV><DIV class="MsoNormal"><SPAN style=""><SPAN style="mso-spacerun: yes"><SPAN class="Apple-style-span" style="font-size: 24px;"><FONT class="Apple-style-span" face="Chalkboard"><FONT class="Apple-style-span" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"> </SPAN></FONT></FONT></SPAN></SPAN><SPAN class="Apple-style-span" style="font-size: 24px;"><FONT class="Apple-style-span" face="Chalkboard"><FONT class="Apple-style-span" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">I tried to create a topology file for DMPC (based on the 53A6 parameters) and encounter some problems, I wonder if someone already did it and can share the file.</SPAN></FONT></FONT></SPAN><FONT class="Apple-style-span" size="6"><SPAN class="Apple-style-span" style="font-size: 24px;"><FONT class="Apple-style-span" face="Century"><FONT class="Apple-style-span" face="Calisto MT"><FONT class="Apple-style-span" face="Century Gothic"><FONT class="Apple-style-span" face="Century Schoolbook"><FONT class="Apple-style-span" face="Chalkboard"><FONT class="Apple-style-span" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"><O:P></O:P></SPAN></FONT></FONT></FONT></FONT></FONT></FONT></SPAN></FONT></SPAN></DIV><DIV class="MsoNormal"><SPAN style=""><SPAN style="mso-spacerun: yes"><SPAN class="Apple-style-span" style="font-size: 24px;"><FONT class="Apple-style-span" face="Chalkboard"><FONT class="Apple-style-span" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"> </SPAN></FONT></FONT></SPAN></SPAN><SPAN class="Apple-style-span" style="font-size: 24px;"><FONT class="Apple-style-span" face="Chalkboard"><FONT class="Apple-style-span" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">Thanks.</SPAN></FONT></FONT></SPAN></SPAN></DIV><DIV><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"><BR></SPAN></FONT></FONT><DIV> <P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"><BR class="khtml-block-placeholder"></SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"><BR class="khtml-block-placeholder"></SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">===========================================</SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| Itamar Kass</SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| The Alexander Silberman</SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| Institute of Life Sciences</SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| Department of Biological Chemistry</SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| The Hebrew University, Givat-Ram</SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| Jerusalem, 91904, Israel</SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| Tel: +972-(0)2-6585194</SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| Fax: +972-(0)2-6584329</SPAN></FONT></FONT></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| Email: </SPAN></FONT></FONT><A href="mailto:ikass@cc.huji.ac.il"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">ikass@cc.huji.ac.il</SPAN></FONT></FONT></A></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">| Web: </SPAN></FONT></FONT><A href="http://munch.ls.huji.ac.il/~membranelab/itamar/itamar_homepage.html"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">http://munch.ls.huji.ac.il/~membranelab/itamar/itamar_homepage.html</SPAN></FONT></FONT></A></P><P style="margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica"><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;">============================================</SPAN></FONT></FONT></P><DIV><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"><BR class="khtml-block-placeholder"></SPAN></FONT></FONT></DIV><DIV><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"><BR class="khtml-block-placeholder"></SPAN></FONT></FONT></DIV> </DIV><FONT class="Apple-style-span" color="#000000"><FONT class="Apple-style-span" face="Chalkboard" size="4"><SPAN class="Apple-style-span" style="font-size: 14px;"><BR></SPAN></FONT></FONT></DIV></BODY></HTML>