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<DIV><FONT face="Times New Roman" color=#000080 size=2>Dear David,</FONT></DIV>
<DIV><FONT face="Times New Roman" color=#000080 size=2></FONT> </DIV>
<DIV><FONT face="Times New Roman" color=#000080 size=2> Thanks
so much for your reply on Zn - His-NE2 restraint.</FONT></DIV>
<DIV><FONT face="Times New Roman" color=#000080 size=2></FONT> </DIV>
<DIV><FONT face="Times New Roman" color=#000080
size=2> You told me to restrain the bonds between
zinc and two His-NE2 for the zinc protease MD simulation, however, I would like
to make sure </FONT></DIV>
<DIV><FONT face="Times New Roman" color=#000080 size=2>whether I correctly set
up the restraint, so that I can SAFELY perform the
production run.</FONT><FONT face="Times New Roman" color=#000080 size=2> I
simply added force on three atoms: Zn and two His-NE2, is this OK??
</FONT></DIV>
<DIV><FONT face="Times New Roman" color=#000080 size=2></FONT> </DIV>
<DIV><FONT face="Times New Roman" color=#000080 size=2>Thanks
again!!</FONT></DIV>
<DIV><FONT face="Times New Roman" color=#000080 size=2></FONT> </DIV>
<DIV><FONT face="Times New Roman" color=#000080
size=2>Linda</FONT></DIV></BODY></HTML>