<DIV>Hi everybody,</DIV> <DIV> </DIV> <DIV>I try to calculate a protein which has two chains.This chains has sulphide bridges between eachother.I used -merge in pdb2gmx.I calculated for 20ns.I said free energy:no.</DIV> <DIV> </DIV> <DIV>But I got this error:</DIV> <DIV> </DIV> <DIV><EM>Fatal error:<BR></EM>><I> Too many LINCS warnings (10716) - aborting to avoid logfile runaway.<BR></I>><I> This normally happens when your system is not sufficiently <BR></I>><I> equilibrated,or if yo<BR></I>><I> u are changing lambda too fast in free energy simulations.<BR></I>><I> If you know what you are doing you can adjust the lincs warning threshold<BR></I>><I> in your mdp file, but normally it is better to fix the problem.<BR></I>><EM> <BR>I looked at mailing list.Idecided to change </EM>delta-lambda=0.00002 </DIV> <DIV>Can anyone help me about this?</DIV> <DIV> </DIV> <DIV> </DIV> <DIV>Thank
you</DIV><p> 
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