<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<HTML><HEAD>
<META http-equiv=Content-Type content="text/html; charset=gb2312">
<META content="MSHTML 6.00.2900.3059" name=GENERATOR>
<STYLE></STYLE>
</HEAD>
<BODY bgColor=#ffffff>
<DIV><FONT size=2><FONT size=3>Hello,everyone.I want to simulate a membrane
protein using <BR>inflategro to construct the initial structure,( <A
href="http://moose.bio.ucalgary.ca/index.php?page=Translate_lipdis">http://moose.bio.ucalgary.ca/index.php?page=Translate_lipdis</A>
)<BR>and I did the job like this:<BR><BR>First I got the equalibrated structure
of POPC bilayer from Tileman's website,put 1RHZ<BR>right at the centre of lipid
bilayer.Then the coordinates of protein were rstricted, and the<BR>coorinates of
lipids were rescaled by a factor 4, next I cut off all the lipids within a
distance of 14 A from protein ,rescaled lipids coordinates by 0.95,and cut off
lipids within 14A from protein.Then I restriction the position of protein and
minize the energy of lipids.However the minimization always crash.<BR><BR>So
will somebody please tell me how to construct the initial simulation
structure of membrane protein using "dig hole" method in detail? and
why the em crash? I have tried all the methods I could find but they
just didn't work.You are very thankful to offer some
help.</FONT><BR></FONT></DIV></BODY></HTML>