<html>
<head>
<style>
.hmmessage P
{
margin:0px;
padding:0px
}
body.hmmessage
{
FONT-SIZE: 10pt;
FONT-FAMILY:Tahoma
}
</style>
</head>
<body class='hmmessage'>
<BR><BR><BR>
<STYLE>
.ExternalClass .EC_hmmessage P
{padding:0px;}
.ExternalClass body.EC_hmmessage
{font-size:10pt;font-family:Tahoma;}
</STYLE>
<P class=EC_EC_MsoNormal><SPAN lang=EN-GB><FONT size=3><FONT color=#000000><FONT face="Times New Roman">Hello,</FONT></FONT></FONT></SPAN></P>
<P class=EC_EC_MsoNormal><SPAN lang=EN-GB><FONT size=3><FONT color=#000000><FONT face="Times New Roman">I am a new user of Gromacs, and I am trying to simulate a system of a membrane composed by POPC, cholesterol and sphingomyelin and a protein. I am using lipid.itp, but it includes ffgmx.itp, and I have read it is deprecated, so I am having a lot of problems to introduce the protein topology with ffG43a1 or opls, without using ffgmx.itp also for the protein. </FONT></FONT></FONT></SPAN></P>
<P class=EC_EC_MsoNormal><SPAN lang=EN-GB><FONT size=3><FONT color=#000000><FONT face="Times New Roman">I would like to know which is the recommendation, because I am a bit confused about this, an in the case of using a different force field from the deprecated ffgmx for the protein, does anybody know where I could get the paremeters for the Berger lipids constructed with a more modern forcefield than ffgmx?</FONT></FONT></FONT></SPAN></P>
<P class=EC_EC_MsoNormal><SPAN lang=EN-GB><FONT size=3><FONT color=#000000><FONT face="Times New Roman">I hope somebody could help me, I will really appreciate your advices.</FONT></FONT></FONT></SPAN></P>
<P class=EC_EC_MsoNormal><SPAN lang=EN-GB><FONT size=3><FONT color=#000000><FONT face="Times New Roman">Best wishes, </FONT></FONT></FONT></SPAN></P>
<P class=EC_EC_MsoNormal><SPAN lang=EN-GB><FONT face="Times New Roman" color=#000000 size=3> </FONT></SPAN></P>
<P class=EC_EC_MsoNormal><FONT face="Times New Roman" color=#000000 size=3>Rebeca Garcia</FONT></P>
<P class=EC_EC_MsoNormal><FONT face="Times New Roman" color=#000000 size=3>Parc Cientific de Barcelona </FONT></P>
<P class=EC_EC_MsoNormal><FONT face="Times New Roman" color=#000000 size=3>Universidad de Barcelona</FONT></P>
<P class=EC_EC_MsoNormal><FONT face="Times New Roman" color=#000000 size=3>regafan@hotmail.com</FONT></P><BR>
<HR>
Sigue de cerca las últimas tendencias y lo que más rompe <A href="http://video.msn.com/video.aspx?mkt=es-es" target=_blank>MSN Vídeo</A> <BR>
<HR>
Todo ruedas: información práctica y todo el glamour del mundo del motor. <A href="http://estilo.es.msn.com/" target=_blank>MSN Estilo y Tendencias</A> <br /><hr />Sigue los principales acontecimientos deportivos en directo. <a href='http://video.msn.com/video.aspx?mkt=es-es' target='_new'>MSN Motor</a></body>
</html>