<table cellspacing="0" cellpadding="0" border="0" ><tr><td valign="top" style="font: inherit;"><DIV><BR>my procesure:</DIV>
<DIV>1. cat cgprotein cgmembrane>system.gro (using Martini force field)</DIV>
<DIV>2 perl inflategro.pl system.gro 4 POPC 14 system_inflated.gro 5 area.dat<BR>3. grompp_d -f em.mdp -c system_inflated.gro -p topol.top -o system_inflatedem.tpr (with strong postion restraint)</DIV>
<DIV>4. mdrun</DIV>
<DIV>5 in the system_inflatedem.gro , the protein and membrane separate each other.</DIV>
<DIV>please help me !</DIV>
<DIV> <BR><BR><A class=plink href="http://cn.webmessenger.yahoo.com/index.php?t=1&to=eWlkPXpoYW94aWl0YzIwMDI-&sig=703fa929658518b2720b087c59cd85f2dabf8844" rel=nofollow target=_blank><IMG class=pimg border=0 alt=4 src="http://opi.yahoo.com/online?u=zhaoxiitc2002&t=4&l=cn"></A><BR><BR>--- <B>10年4月12日，周一, Justin A. Lemkul <I><jalemkul@vt.edu></I></B> 写道：<BR></DIV>
<BLOCKQUOTE style="BORDER-LEFT: rgb(16,16,255) 2px solid; PADDING-LEFT: 5px; MARGIN-LEFT: 5px"><BR>发件人: Justin A. Lemkul <jalemkul@vt.edu><BR>主题: Re: [gmx-users] construct CG membrane protein and CG membrane and inflategro.pl<BR>收件人: "Discussion list for GROMACS users" <gmx-users@gromacs.org><BR>日期: 2010年4月12日,周一,下午8:42<BR><BR>
<DIV class=plainMail><BR><BR>xi zhao wrote:<BR>> <BR>> <BR>> Dear Gromacs users:<BR>> I use inflateGRO.pl to insert CG protein in to CG POPC membrane, but when carryied EM with Strong POStion REStraint, the protien and membrane were apart. Please help me!<BR><BR>What does "apart" mean? If you want free help, you have to make it easy to help you. That means thorough descriptions of what you're doing, what you're seeing, and if necessary, posting images online (not as attachments!) for us to take a look. The InflateGRO method should generate a configuration that has molecules separated by a large amount of space, but if there is something else wrong you'll have to provide a better description.<BR><BR>-Justin<BR><BR>> 4 <<A href="http://cn.webmessenger.yahoo.com/index.php?t=1&to=eWlkPXpoYW94aWl0YzIwMDI-&sig=703fa929658518b2720b087c59cd85f2dabf8844"
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