<div>Dear Justin</div>
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<div>thanks for your attention.</div>
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<div>You said that separation of two strands of DNA during of simulation is a normal consequence of periodic boundary conditions. I had used periodic boundary condition in mdp file as following:</div>
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<div>Periodic boundary conditions:<br>pbc              = xyz</div>
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<div>Should I delete pbc from mdp file?</div>
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<div><span class="short_text" id="result_box"><span title="" style="COLOR: #000; BACKGROUND-COLOR: #e6ecf9">Whether elimination of pbc prevents separation of two strand of DNA?</span></span></div>
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<div><span class="short_text"><span title="" style="COLOR: #000; BACKGROUND-COLOR: #e6ecf9">SS</span></span></div>