<html><head><style type="text/css"><!-- DIV {margin:0px;} --></style></head><body><div style="font-family:times new roman,new york,times,serif;font-size:12pt"><div>Dear All,<br> I have pulled a protein by keeping n-terminus fixed and pulling the c-terminus. I tried to extract the force.xvg profile from the .trr file by the following command -<br><br>g_traj -f traj.trr -s pull.tpr -of force.xvg<br><br>But in the force.xvg profile I am getting both positive and negative values of force. What does it mean? Please suggest.<br>Samrat <br></div>
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