<span class="Apple-style-span" style="font-family: Helvetica; font-size: medium; ">Hi,</span><span class="Apple-style-span" style="font-family: Helvetica; font-size: medium; "> </span><span class="Apple-style-span" style="font-family: Helvetica; font-size: medium; "><br>
</span><span class="Apple-style-span" style="font-family: Helvetica; font-size: medium; ">I am using pdb2gmx with amber ff and there is a problem adding the H atom to the second residue of both chains of my protein? The residue at that location is repeated in later in the chain and has no problem with the hydrogens so I don't understand why it gives me the error?</span><span class="Apple-style-span" style="font-family: Helvetica; font-size: medium; "><br>
</span><span class="Apple-style-span" style="font-family: Helvetica; font-size: medium; "><br></span><span class="Apple-style-span" style="font-family: Helvetica; font-size: medium; ">I relatively new to this and this is the first time I'm using amber ff in gromacs so thanks for all the previous posts which help me quite a bit so far.</span><span class="Apple-style-span" style="font-family: Helvetica; font-size: medium; "><br>
</span><span class="Apple-style-span" style="font-family: Helvetica; font-size: medium; ">Stacey</span>