<html>
<head>
<style><!--
.hmmessage P
{
margin:0px;
padding:0px
}
body.hmmessage
{
font-size: 10pt;
font-family:Tahoma
}
--></style>
</head>
<body class='hmmessage'>
<font class="Apple-style-span" face="Tahoma">Hello,</font><div style="font-family: Tahoma; "><br></div><div style="font-family: Tahoma; ">I am attempting to add 8-oxo-dG (<span class="Apple-style-span" style="font-family: arial, sans-serif; font-size: 10pt; line-height: 15px; ">8-Oxo-2'-deoxyguanosine) to the amber99sb force field. This is a simple modification of the H8 hydrogen on a guanine to an oxygen atom. I have followed the instructions on the gromacs site on adding residues.</span></div><div style="font-family: Tahoma; "><span class="Apple-style-span" style="font-family: arial, sans-serif; font-size: 10pt; line-height: 15px; "><br></span></div><div style="font-family: Tahoma; "><span class="Apple-style-span" style="font-family: arial, sans-serif; font-size: 10pt; line-height: 15px; ">However the new nucleotide will not work, it will not attach to the adjacent nucleotides in the sequence.</span></div><div><font class="Apple-style-span" face="arial, sans-serif"><span class="Apple-style-span" style="line-height: 15px;"><br></span></font></div><div><font class="Apple-style-span" size="2"><span class="Apple-style-span" style="font-size: 10pt; "></span></font><font class="Apple-style-span" face="arial, sans-serif"><span class="Apple-style-span" style="line-height: 15px;">Any help would be very much appreciated, if any more information is required please let me know.</span></font></div><div><font class="Apple-style-span" face="arial, sans-serif"><span class="Apple-style-span" style="line-height: 15px;"><br></span></font></div><div><font class="Apple-style-span" face="arial, sans-serif"><span class="Apple-style-span" style="line-height: 15px;">Best Regards</span></font></div><div><font class="Apple-style-span" face="arial, sans-serif"><span class="Apple-style-span" style="line-height: 15px;"><br></span></font></div><div><font class="Apple-style-span" face="arial, sans-serif"><span class="Apple-style-span" style="line-height: 15px;">William Stebbeds</span></font></div><div><font class="Apple-style-span" face="arial, sans-serif"><span class="Apple-style-span" style="line-height: 15px;">Cranfield University</span></font></div><div><font class="Apple-style-span" face="arial, sans-serif"><span class="Apple-style-span" style="line-height: 15px;">UK</span></font></div><div><br></div> </body>
</html>