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Hello,<br>I am trying to calculate the binding energy between two monomers in three different dimers, using PMF (Umbrella Sampling method) and following Justin's tutorial.<br>Using 100 windows separated 0.05 nm I get the PMFs represented in "pmf_using_100_points.pdf" (attached), and using 50 windows separated 0.1 nm I get different PMF results, represented in "pmf_using_50_points.pdf"<br>How is it possible to obtain so different results depending on the number of windows used in the Umbrella Sampling Calculation?<br>Any help is appreciated. <br>Thanks a lot in advance!<br>Best wishes,<br>Rebeca.<br><br>Dr. Rebeca Garcia <br>Santiago de Compostela University<br>Spain<br><br> </div></body>
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