<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div>Hi,</div><div><br></div><div>The genion command i used is</div><div>genion -s genion.tpr -o ago_water_ions.gro -nn 6</div><div><br></div><div>I used this to add 6 CL ions and i got the following error:</div><div> System has non-zero total charge: 5.700000e+01<br></div><div><br></div><div>So i should add another 5 CL ions to neutralize the system?</div><div><br></div><div><br></div><div>Many thanks,</div><div>Munishika</div></div></body></html>