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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hi guys,<br>
<br>
I was just wondering if with "direction_periodic" the pull simulations pulls at both ends of the protein rather than just one.<br>
<br>
I've previously been position restraining the C-terminus of my protein and pulling on the N-terminus. With the "distance" pull code this seems to do exactly that - hold the C- and pull the N- away from it. Changing over to the "direction_periodic" it seems
that the force is being applied to both the C- and N- termini even though the position restraint file has been specified when creating the .tpr with grompp ... is this right? How can I stop it from pulling on both termini if this is in fact the case?<br>
<br>
Thanks<br>
Natalie<br>
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<span style="font-weight: bold;">Natalie Stephenson, B.Sc</span><br style="font-weight: bold;">
<span style="font-weight: bold;">PhD Research Associate</span><br>
<br>
Manchester Interdisciplinary Biocentre<br>
131 Princess Street<br>
Manchester<br>
M1 7DN<br>
x65816<br>
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