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On 11/11/2011 12:05 AM, LindaSong wrote:
<blockquote
cite="mid:4fe9be18.1dd7a.1338d953957.Coremail.wlsonglinda@gmail.com"
type="cite">
<div
style="line-height:1.7;color:#000000;font-size:14px;font-family:arial"> Hello
all,<br>
I want to use g_membed to insert a protein in POPC. I followed
the manual steps. I used the grompp to generate the tpr file. It
works well. But when I handed this tpr file to g_membed, it
returned this:<br>
-------------------------------------------------------<br>
Program g_membed, VERSION 4.5.3<br>
Source code file: gmx_membed.c, line: 688<br>
<br>
Fatal error:<br>
Trying to remove more lipid molecules than there are in the
membrane<br>
-------------------------------------------------------<br>
Why it tries to remove more lipid molecules than there are in
the membrane? <br>
The membrane has 256 lipid molecules. I used PyMol to see the
structure of the protein with POPC. The lipids cover the whole
protein. <br>
I don't know what's wrong here.<br>
</div>
</blockquote>
<br>
We can't know, because you haven't given us information like command
lines and output copied from your terminal so we can see the
diagnostic information g_membed printed for you.<br>
<br>
Mark<br>
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