<p class="MsoNormal"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Dear GROMACS users,</span></p>

<p class="MsoNormal"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"><br></span></p><p class="MsoNormal"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Total charge of my system:</span></p>


<p class="MsoNormal" style="margin-bottom:0.0001pt;text-indent:36pt"><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Including chain
1 in system: 2041 atoms 207 residues</span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;text-indent:36pt"><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Including chain
2 in system: 2036 atoms 206 residues</span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;text-indent:36pt"><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Including chain
3 in system: 44 atoms 1 residues</span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;text-indent:36pt"><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Now there are
4121 atoms and 414 residues</span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;text-indent:36pt"><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Total mass in
system 46007.483 a.m.u.</span></p>

<p class="MsoNormal" style="margin-bottom:0.0001pt;text-indent:36pt"><b><span style="font-size:12.0pt;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Total charge in system 0.748 e</span></b></p>

<p class="MsoNormal"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"> </span></p>

<p class="MsoNormal"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Is it possible to make charge in system zero and how?</span></p>

<p class="MsoNormal"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"><br></span></p><p class="MsoNormal"><span style="font-size:12.0pt;line-height:115%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Thanks in advance for your reply</span></p>