<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div>Dear justin,,</div><div> Thank you for your immediate reply.</div><div>Which PDB is suitable Either X-ray PDB (brookavean protein data bank) or NMR PDB to study the effect of ionic strength on protein.</div><div>If i use x-ray PDB <br></div><div>will it affect the result though i solvate and neutralise the PDB before energy minimization, Equilibration and MD ?</div><div>Thanks in advance <br></div></div></body></html>