<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div>Dear justin, Thanks for your previous reply<br></div><div><br></div><div>which one is reliable to get desired spacing in umbrella sampling either from output of g_dist or from pullx.xvg?</div><div><br></div><div>If i choose spacing from pullx.xvg will it affect the result ? (poor sampling and poor free energy calculation). <br></div><div>Thanks in Advance<br></div><div></div></div></body></html>