Peter,<br><br>thank you it's very intresting survey. I'll try to
investigate it carefully. I've looking for the same issue for along time
it's also would be good to find more recent review on the same topic.<br>
<br>By the way today I've found that the testing on the receptor
stability in the case of some point mutations might not be good with the
unbiassed MD simulation. Some people told me that for most valid result
the biased MD by the aplication of the external forces must be done.
Finally even if i've done very 'deadly' mutation (e.g strong
perturbation of the tight packing in the functional-relevant region of
the transmembrane segment) this effect could not been seen even in the
relatively long trajectory.<br>
<br>By contract that publication wich you provide the different
conditions were tested for the refirement of the X-ray result in the
standart MD. <br><br>So is there any compromise between both of the methods of the MD?<br><br>James<br>