Dear Gromacs users!<br><br><br>I've constructed my biphastic system with the water-ccl4-water layers where in the ccl4 layer I've placed my membrane protein.<br><br>Now I'd like to place addition 5 Cl ions to the bottow leafleat of water to mimick the 'positive-inside rule' (my protein consist of 5 Lys) of the membrane protein topology. How I could specify to place some ions in the desired possitions? As i understood the genion place ions in the random possitions so I've obtained 3 ions in the bottom and 2 in the upper layer.<br>
<br>Thanks<br><br>James<br>