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On 1/03/2012 11:57 AM, Dialing Pretty wrote:
<blockquote
cite="mid:1330563442.2389.YahooMailNeo@web121702.mail.ne1.yahoo.com"
type="cite">
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<div>Dear All,</div>
<div> </div>
<div>Some of the complexes of PDB files in RCSB are not from
protein-protein interaction, but from the packing of protein
crystal. Thus in solution the "protien complex" may
dissociate.</div>
<div> </div>
<div>However as for the protein are usually very large, and I
regard it is difficult to observe that 2 (or more) subunit
protein complex dissociate completely during MD simulation.</div>
<div> </div>
<div>Can you please introduce to me your experience that the
"protein complex" sifnificantly dissociate during MD<var
id="yui-ie-cursor"></var>?</div>
</div>
</blockquote>
<br>
That's a very expensive way to observe whether the complex is
thought to be "real" or not - if it is possible to get a reliable
result. Reading the literature that led to the PDB contribution and
about the protein generally will be much more productive.<br>
<br>
Mark<br>
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