<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div style="RIGHT: auto">Dear All,</div>
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<div style="RIGHT: auto">I remember before I have read a something on melting the protein from a predefined starting temperature to a predefined end temperature by MD, so that after the MD we can look at the whole process to see which part of the protein unfold earlier, which part of the protein of the protein unfold earlier. No I cannot find that introduction.<VAR id=yui-ie-cursor></VAR></div>
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<div style="RIGHT: auto">After we equilibriate the protein system, will you please tell me how to define the temperature gradient from the start temperature to the end temperature in the mdp file? Or do you have a protocol on it?</div>
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<div style="RIGHT: auto">Cheers,</div>
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<div style="RIGHT: auto">Fenghui</div></div></body></html>