<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><br> <div style="font-family: times new roman,new york,times,serif; font-size: 12pt;">Dear All,<br><br>For the on-line Umbrella Sampling tutorial "http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin/gmx-tutorials/umbrella/04_EM_equil.html" of <font face="Arial"><b>Justin Lemkul</b></font>, will you pleasse tell me for the equilibration where there is no NVT equilibration?<br><br>Cheers,<br><br>Acoot<br> </div> </div></body></html>