<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div><br> Dear All,</div><div> </div><div>I have a protein, the charged residue numbers in the protein is as following</div><div>ARG 11<br>ASP 19<br>glu 25<br>his 8<br>lys 24</div><div> </div><div>asp+glu= 44<br>arg+his+lys=43</div><div> </div><div>However the total charge of the protein given by pdb2gmx is -9.</div><div> </div><div>Will you please introduce to me how pdb2gmx calculate the total charge of the protein?</div><div> </div><div>Cheers,</div><div> </div><div>Acoot</div> </div></body></html>