Hi ALL,<div><br></div><div>I am simulating a membrane protein docked with a ligand and embedded in a lipid bilayer. For COM removal I am using two groups, "Prt_Lig_Lipid" and "SOL_CL". For temperature and pressure couplings should I use these two groups or should I use three groups, "Protein", "Lipid" and "Lig_SOL_CL"?</div>
<div>Any suggestion is welcome.</div><div><br></div><div><br></div><div>Thanks and regards,</div><div><br></div><div>Anirban</div>